Vol. 8, No 19, p. 803-809 - 31 ago. 2021
Expressão relativa do gene OsNPF7.4 em arroz é estimulada pelo fornecimento de nitrato em solução nutritiva
Erika da Costa Fernandes
,
Maria Eduarda Pimentel de Melo
Erinaldo Gomes Pereira ,
Brisa Ribeiro de Lima ,
Manlio Silvestre Fernandes e
Leandro Azevedo Santos Resumo
O nitrato (NO3-) é a forma predominante de nitrogênio em solos bem aerados. Estudos recentes apontam a importância do gene OsNPF7.4 na eficiência de uso de N em arroz, atuando diretamente na multiplicação dos perfilhos. Entretanto, estudos de expressão desse gene sob variação no fornecimento de nitrato ainda são escassos. O objetivo do presente trabalho foi a caracterização da resposta deste gene frente a um período de supressão de nitrato (N-N3-) de 72 h. Plantas de arroz da variedade manteiga foram cultivadas em solução nutritiva por 30 dias e então submetidas a um período de supressão de N por 72 h, seguido pelo reestabelecimento do nutriente. Foram realizadas coletas do tecido da bainha a cada 24 h no período de supressão e nos tempos 4, 8, 24 e 72 h após o ressuprimento e realizou-se análise da expressão relativa por PCR em tempo real. O gene OsNPF7.4 apresenta duas isoformas, derivadas de splicing alternativo. Com a análise da expressão relativa foi observado um decréscimo dos níveis de resposta das duas isoformas do gene no período de supressão de N e um pico de expressão logo após o ressuprimento. Estes resultados confirmam a alta dependência deste gene quanto a presença de NO3- na solução e indicam sua possível atuação no influxo de nitrato.
Palavras-chave
Nitrato; Influxo; Transportador NPF.
Abstract
Relative expression of OsNPF7.4 gene is stimulated by nitrate supply. Nitrate
(NO3-) is the predominant form of nitrogen in well-aerated soils.
Recent studies show the importance of OsNPF7.4 gene on N use efficiency in rice,
acting directly in the tillering. However, the characterization of this gene expression
under different conditions of nitrate supply is still scarce. The aim of this study was to
characterize the response of OsNPF7.4 to a nitrate (N-NO3-)
suppression period. Rice plants, "manteiga" variety was cultivated in nutrient
solution subjecting them a N suppression for 72 h, followed by the reestablishment. The
sheaths were collected every 24 h during the suppression period and 4, 8, 24 and 72 h after
resupply. Then real time PCR analysis was used to study its relative expression. The gene
OsNPF7.4 has two isoforms originated by alternative splicing. Relative expression
reveals that both of them show a decrease in the response of transcription after suppression
of N (succeeding) followed by an increase after resupply. These results confirm the high
dependence of this gene (regarding) on the presence of NO3- in solution
and indicate its possible performance in nitrate influx.
Keywords
Nitrate; Influx; NPF transporter.
DOI
10.21438/rbgas(2021)081910
Texto completo
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https://doi.org/10.1093/jxb/eru425
ISSN 2359-1412