Effects of temperature and storage period on the activity of L-asparaginase produced by a local isolate of Bacillus licheniformis

Vivian Daifallah , Sabah Yazaji e Lina Al-Amir

Abstract
L-asparaginase enzyme efficacy is affected by storage conditions and thus further studies are required to determine optimum temperatures and storage conditions to maintain L-asparaginase activity. L-asparaginase produced by Bacillus licheniformis, isolated from Syrian soils, was stored at different temperatures (-20 °C, 4 °C, 10 °C, 30 °C, and 60 °C) for 1 year (8,640 hrs), where the enzymatic activity was measured at several time intervals (24, 48, 120, 288, 480, 720, 1,512, 4,320, and 8,640 hrs). The results showed that the enzyme maintained 95.5% of its activity at the end of the storage period (1 year) when a temperature of -20 °C was used. At the other temperatures, a significant reduction in enzyme activity was observed after 24 hrs of storage.

Keywords
Asparaginase; Bacillus licheniformis; Storage; Temperature; Asparagine.

Resumo
Efeito da temperatura e do período de armazenamento na atividade da L-asparaginase produzida por um isolado local de Bacillus licheniformis. A eficácia da enzima L-asparaginase é afetada pelas condições de armazenamento e, portanto, são necessários mais estudos para determinar temperaturas e condições de armazenamento ideais para manter a sua atividade. A L-asparaginase produzida pelo Bacillus licheniformis, isolada de solos sírios, foi armazenada em diferentes temperaturas (-20 °C, 4 °C, 10 °C, 30 °C e 60 °C) por um ano (8.640 h), onde a atividade enzimática foi medida em vários intervalos de tempo (24, 48, 120, 288, 480, 720, 1.512, 4.320 e 8.640 h). Os resultados mostraram que a enzima manteve 95,5% de sua atividade ao final do período de armazenamento (1 ano) quando foi utilizada a temperatura de -20 °C. Nas demais temperaturas foi observada redução significativa na atividade enzimática após 24 h de armazenamento.

Palavras-chave
Asparaginase; Bacillus licheniformis; Armazenamento; Temperatura; Asparagina.

DOI
10.21438/rbgas(2023)102615

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