Condições de luz sob o rendimento de biocompostos funcionais primários nas espécies de microalgas Limnospira maxima e Chlorella vulgaris

Rosy Dayanne Fernandes Nascimento , Janaina da Silva Gomes , Fabiana Rodrigues de Arruda Câmara e Dárlio Inácio Alves Teixeira

Resumo
O uso de diferentes fontes de luz tem sido uma estratégia eficiente no aumento da produção e no acúmulo de biomoléculas microalgal. O objetivo desta pesquisa foi analisar o efeito da exposição a lâmpadas brancas LED e fluorescente no rendimento de biocompostos primários das espécies de microalgas Limnospira maxima e Chlorella vulgaris. As espécies foram cultivadas em bombonas de 4 L sob fotoperíodo 12 h:12 h e controle de pH, salinidade e temperatura. Foram avaliados durante o período experimental a densidade óptica, absorbância, peso seco e contagem de célula. Para quantificação de proteínas, lipídios e carboidratos totais foram utilizadas metodologias específicas, de acordo com a literatura. Os resultados mostraram, através do número de células, que os cultivos expostos à luz LED branca apresentou maior acúmulo de biomassa para ambas as espécies. O teor de proteínas foi significativamente maior na luz LED para Limnospira maxima (45,13 ± 2,232), comparado com Chlorella vulgaris o maior percentual foi na luz fluorescente (19,97 ± 0,6411). Não houve diferença significativa entre os teores de carboidratos em ambas as espécies. Os teores de lipídios da Limnospira maxima foram significativamente maiores na luz LED branca (17,77 ± 1,254) e Chlorella vulgaris apresentou maior rendimento na luz fluorescente (4,10 ± 1,391). Portanto, a luz LED branca é a mais adequada para produção de biomassa, proteínas e lipídios em Limnospira maxima e a Chlorella vulgaris, exposta à luz fluorescente, apresentou melhor e maior adaptação e rendimento microalgal.

Palavras-chave
Microalgas; LED; Luz fluorescente; Biomoléculas; Intensidade luminosa.

Abstract
Light conditions under the yield of primary functional biocompounds in the microalgae species Limnospira maxima and Chlorella vulgaris. The use of different light intensities has been an efficient strategy in increasing the production and accumulation of microalgal biomolecules. The objective of this paper was to analyze the effect of the manipulation of light intensities on the production of crops under the yield of primary functional biocompounds of the species of microalgae Limnospira maxima and Chlorella vulgaris. The strains of the crops were produced in the laboratory under the following conditions: photoperiod, pH, aesthetion, optical density, absorbance, salinity, dry weight and cell count. For quantification of proteins, lipids and total carbohydrates were followed according to the literature. The results showed that the average number of cells of the cultures exposed in the white LED light showed better and higher biomass accumulation for both species. For carbohydrates, Chlorella vulgaris (26.74 ± 4.64) and Limnospira maxima (22.84 ± 11.07) presented statistically equal values between treatments. In proteins, the best result was in white LED light in the Limnospira maxima (45.13 ± 2.232), presenting significant difference. The lipid contents of Limnospira maxima were significantly higher in white LED light (17.77 ± 1.254) and Chlorella vulgaris showed higher yield in fluorescent light (4.10 ± 1.391). Given this, we highlight, that white LED light is the main color for the cultivation and maintenance of Cyanobacteria and microalgae.

Keywords
Microalgae; LED light; Fluorescent light; Biomolecules; Luminous intensity.

DOI
10.21438/rbgas(2024)112819


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